Multivariate analysis and genetic diversity for morphometric and root textural quality traits in ashwagandha (Withania somnifera Dunal)

Ashwagandha (Withania somnifera) is an important medicinal plant and its dried roots are used in traditional systems of medicine. The market price of roots is determined by physical (textural) quality. Brittle roots with high starch and low fiber are considered to be superior because of ease in grinding. Genetic diversity studies based on root textural parameters have not been done so far. So the present study was designed to assess genetic diversity for morphometric traits and root textural quality parameters among two morphologically distinct groups: Poshita and Nagore. The PCA separated the morphometric and root texture variables distinctly into two different principal components: PC-1 and PC-2 respectively, indicating that both are negatively associated. All the morphotypes in Poshita group showed high positive loadings in PC-1 indicating that component genotypes are high root yielding. Nagore morphotypes were low yielding but the root texture was good. Clustering of morphotypes grouped Poshita and Nagore separately with high inter-cluster distances indicating that both groups are highly divergent from each other, suggesting that there is sufficient scope for varietal improvement through hybridization.     

Morphological Basis of Resistance in Cotton to the WhiteflyBemisia Tabaci

The morphological basis of resistance to the whiteflyBemisia tabaci Genn. (Aleyrodidae: Hemiptera) was studied. The plant characters examined were leaf area, thickness of leaf lamina, hair density, hair length, angle of insertion of leaf hair, and density of gossypol glands. Hair density and leaf thickness were positively correlated with the population ofB. tabaci, and a positive correlation was obtained between the adult whitefly population and gossypol glands on stem internodes. Cotton genotype USA-22 (sparsely hairy) was found to be more tolerant toB. tabaci than was genotype USA-13 (velvety hairy). The use of thinner and glabrous leaved cotton varieties is suggested to minimize the whitefly menace in cotton.     

抑菌圈-定殖力双重测定法筛选青枯病生防细菌

 本研究首先用平皿抑菌圈法筛选出55个拮抗青枯菌的细菌菌株,将番茄幼苗在各菌菌悬液中浸根12h后栽种于温室未灭菌的土壤中,结果发现,有22个菌株在幼苗根部的定殖能力较强(终定殖密度大于104 cfu/g根),其中革兰氏阴性土壤细菌占同类菌的86.3%,革兰氏阳性土壤细菌占同类菌的13.0%,10个无致病力产细菌素的青枯菌菌株在根部的终定殖密度均低于104 cfu/g根,其定殖能力弱于致病青枯菌。有17个拮抗菌菌株在番茄幼苗根部的定殖密度超过所有致病菌。温室生防结果证明,抑菌圈-定殖力双重测定法对于筛选生防菌株是一种行之有效的方法。     

麦茎谷蛾生物学特性及防治

麦茎谷蛾在山西南部麦区普遍发生，丘陵旱塬地区为害较重。大发生时，孕穗～灌浆期小麦被害穗率达３０％以上。药剂防治试验表明，３月上旬卵态幼虫孵化时用４０％甲基异柳磷、２０％菊马乳油等进行一次性防治，均有较好的效果。     

浙江蚕区的变化及其发展趋势

本文根据《浙江省蚕桑生产区划》的蚕区划分,分析了各蚕区在浙江蚕桑生产持续发展、徘徊滑坡时的变化,及其变化的特点和原因;并分析了蚕区变化的趋势。     

Morphology of Day-7 Bovine Demi-Embryos and their Post-transfer Viability*

Day 7 bovine embryos were microsurgically bisected and replaced into surrogate zonae pellucidae. They were fixed immediately after bisection and at various intervals of in vitro incubation at 35 °C in modified Dulbecco's medium. At the light microscopical level, the bisected embryos restored the prebisection morphology within 30 min. after splitting. The electron microscopy confirmed these findings, suggesting that day 7 bovine demi-embryos for transfer purposes, should be cultured for 30 min before morphologically evaluated. Eleven pairs of bisected day 7 bovine embryos were transferred to 11 synchronized heifers. The recipient heifers were slaughtered at day 15, and the recovered embryos evaluated. Nine of the demi-embryos developed to morphologically, normal spherical to elongated, embryos.     

葡萄叶片光合速率日间降低内外因调控的研究

土壤水分充足时,葡萄日间光合速率的下降主要归因于CO2叶肉阻力的升高;光照、叶温和大气湿度可以影响气孔对CO2的阻力,但主要通过影响叶肉阻力来影响光合速率。‘巨峰’和‘长相思’葡萄具有不同的光合作用光响应曲线及其特征参数。     

减毒鸡沙门菌疫苗株97A的免疫保护效力试验

减毒沙门菌疫苗株97A免疫鸡经鸡沙门菌强毒株Sg9攻击后,对免疫攻毒组和攻毒对照组鸡进行病理学检查和细菌分离,以进一步评价97A疫苗的免疫保护效力。结果表明,免疫攻毒组鸡肝脏、肺脏和肾脏等实质器官仅有轻到中度的炎症反应,而攻毒对照组鸡则表现出了严重的组织病理学变化,同时减毒鸡沙门菌疫苗株97A能显著地限制强毒株在鸡体内的定居和增殖,显示出了良好的免疫保护效力。     

O型口蹄疫疫苗免疫牛抗体消长动态的LPB-ELISA检测

分别按一次免疫、首免后第15d加强免疫和首免后第60d加强免疫程序，对3组试验牛（每组牛20头）用O型口蹄疫疫苗进行了免疫。免疫后不同时间点用液相阻断ELISA（LPB-ELISA）测定了免疫牛O型口蹄疫抗体效价。结果，首免后第15d加强免疫牛和第60d加强免疫牛抗体水平较高，50％保护牛所占比例分别为56．3％和63．1％，完全受保护的牛所占比例分别为34．3％和29．5％。第60d加强免疫牛的保护率要高于第15d加强免疫牛和一次免疫牛的保护率。结果表明，首免后第60d加强免疫是最理想的免疫程序。     

灌溉对华北农牧交错带无芒雀麦生物学特性的影响

研究了河北坝上地区不同水分处理下无芒雀麦的生物学特性。结果表明,不同水分处理对无芒雀麦的植株高度、分蘖数、叶面积、地上和地下植物量等生物学特性均有不同程度的影响。在9月初刈割前,植株高度顺序为W3>W4>W2>W1,表明较多的灌水有利于植株的生长;叶面积大小顺序与植株高度变化规律相似,叶面积大小顺序为W4>W3>W2>W1,表明较多的灌水利于叶片的增大;对于分蘖数则相反,不同水分处理分蘖数顺序为W1>W2>W3>W4,灌水越多分蘖越少,说明整个生长季里适当的干旱可以促进分蘖;不同水分处理下地上植物量的大小次序为W2>W4>W3>W1,但无显著差异;不同水分处理根系重量的大小次序为W2>W1>W3>W4,说明适当干旱有利于无芒雀麦根系的生长。